Mouse Hematology, A Laboratory Manual

The mouse has become a standard laboratory model organism, particularly for the study of hematopoiesis, the immune system, and inflammation. Although laboratories studying stem cells, blood, and blood-forming tissues have assimilated many new molecular diagnostic methods, the identification of cell lineages through classical light microscopic techniques is often poorly understood and practiced.

Mouse Hematology presents a concise review of conventional methods for the preparation, enumeration, and microscopic examination of blood and blood-forming tissues of the laboratory mouse.

Along with a short laboratory manual featuring detailed protocols, Mouse Hematology includes a DVD of short video demonstrations of the techniques and a poster of blood cell types for easy identification at the microscope.

These rapid, inexpensive assessments can save valuable time and resources essential to the design, development, and interpretation of experiments.

Michael P. McGarry, Cheryl A. Protheroe, and James J. Lee, Mayo Clinic Arizona

Bone Marrow Transplant in Mice

The ability to engraft mice with a hematopoietic system derived from another mouse provides the opportunity to study a variety of cell functions. Genetic disparities between donor and host owing to mutation, gene extinction, overexpression or selected insertions have provided models to unravel pathways of differentiation, function and even pathology. Prerequisite to success, however, is….

Authors:  Mike McGarry Ph.D 

Considerations in the Use of the RS 2000 X-ray Irradiator for Biological Research

The performance goal of small animal irradiation device is to provide a uniform dose throughout the animal so that negative effects of high concentrated dose (skin burn) or non-uniform dose (reduced biological effect) areas can be eliminated. .

Authors: Rad Source

Characterization and Dosimetry of a Practical x-ray Alternative to Self-shielded Gamma Irradiators.  

Abstract:
The Insect Pest Control Laboratory of the Joint FAO/IAEA1 Division of Nuclear Techniques in Food and Agriculture recently purchased an X-ray irradiator as part of their programme to develop the sterile insect technique (SIT). It is a self-contained type with a maximum X-ray beam energy of 150 keV using a newly developed 4π X-ray tube to provide a very uniform dose to the product.

This paper describes the results of our characterization study, which includes determination of dose rate in the centre of a canister as well as establishing absorbed dose distribution in the canister.

Our study indicates that this X-ray irradiator provides a practical alternative to self-shielded gamma irradiators for SIT programmes.

Keywords: X-rays; X-radiation; Dosimetry; Radiation processing; SIT; RS 2400

 

Expression of Common Chromosomal Fragile Site Genes, WWOX/FRA16D and FHIT/FRA3B Is Down Regulated By

Common chromosomal fragile sites are unstable genomic loci susceptible to breakage, rearrangement, and are highly recombinogenic. Frequent alterations at these loci in tumor cells led to the hypothesis that they may contribute to cancer development.

The two most common chromosomal fragile sites FRA16D and FRA3B which harbor WWOX and FHIT genes, respectively, are frequently altered in human cancers. Here we report that environmental carcinogens, ultraviolet (UV) light, and Benzo[a]pyrene diol epoxide (BPDE), significantly downregulate expression of both genes. 

https://onlinelibrary.wiley.com/doi/10.1002/mc.20122/abstract

Authors: Thavathiru, E., Ludes-Meyers, J.H., MacLeod, M.C., Aldaz, C.M., 2005.

PASSIVE AND ACTIVE MECHANISMS TRAP ACTIVATED CD8+ T CELLS IN THE LIVER

The liver is a site where activated CD8(+) T cells are trapped and destroyed at the end of an immune response.

The intrahepatic accumulation of activated murine TCR transgenic CD8(+) T cells was significantly reduced when either ICAM-1 or VCAM-1 was blocked by specific Ab. .

Citation:
John, B., Crispe, I.N., 2004. Passive and active mechanisms trap activated CD8+ T cells in the liver. Journal of Immunology 172, 5222-5229.

Website:
https://www.jimmunol.org/cgi/reprint/172/9/5222

PERICYTES AND ENDOTHELIAL PRECURSOR CELLS: CELLULAR INTERACTIONS AND CONTRIBUTIONS TO MALIGNANCY

Tumor vasculature is irregular, abnormal, and essential for tumor growth. Pericytes and endothelial precursor cells (EPC) contribute to the formation of blood vessels under angiogenic conditions.

As primary cells in culture, pericytes and EPC share many properties such as tube/network formation and response to kinase inhibitors selective for angiogenic pathways..

Citation:
Bagley, R.G., Weber, W., Rouleau, C., Teicher, B.A., 2005. Pericytes and Endothelial Precursor Cells: Cellular Interactions and Contributions to Malignancy. Cancer Res. 65, 9741-9750.

Website:
https://cancerres.aacrjournals.org/cgi/reprint/65/21/9741

SHORT COMMUNICATION: NANOPARTICLE THERMOTHERAPY AND EXTERNAL BEAM RADIATION THERAPY FOR HUMAN PROSTA

 

Tumor vasculature is irregular, abnormal, and essential for tumor growth. Pericytes and endothelial precursor cells (EPC) contribute to the formation of blood vessels under angiogenic conditions. As primary cells in culture, pericytes and EPC share many properties such as tube/network formation and response to kinase inhibitors selective for angiogenic pathways.

Expression of cell surface proteins including platelet-derived growth factor receptor, vascular cell adhesion molecule, intercellular adhesion molecule, CD105, desmin, and neural growth proteoglycan 2 was similar between pericytes and EPC, whereas expression of P1H12 and lymphocyte function-associated antigen-1 clearly differentiates the cell types. .

Citation:
Bagley, R.G., Weber, W., Rouleau, C., Teicher, B.A., 2005. Pericytes and Endothelial Precursor Cells: Cellular Interactions and Contributions to Malignancy. Cancer Res. 65, 9741-9750.

Website:
https://cancerres.aacrjournals.org/cgi/reprint/65/21/9741

 

DERIVATION OF LUNG EPITHELIUM FROM HUMAN CORD BLOOD-DERIVED MESENCHYMAL STEM CELLS

Recent studies have suggested that both embryonic stem cells and adult bone marrow stem cells can participate in the regeneration and repair of diseased adult organs, including the lungs. However, the extent of airway epithelial remodeling with adult marrow stem cells is low, and there are no available in vivo data with embryonic stem cells.

Human umbilical cord blood contains both hematopoietic and nonhematopoietic stem cells, which have been used clinically as an alternative to bone marrow transplantation for hematologic malignancies and other diseases..

Authors: 

Citation:
Am J Respir Crit Care Med. 2008 Apr 1;177(7):701-11. Epub 2007 Dec 6.

Website:
https://ajrccm.atsjournals.org/cgi/content/full/177/7/701

 

THE APPLICATION OF RADIATION THERAPY TO THE PEDIATRIC PRECLINICAL TESTING PROGRAM

The Pediatric Preclinical Testing Program (PPTP) has been successfully used to determine the efficacy of novel agents against solid tumors by testing them within a mouse-flank in vivo model.

To date, radiation therapy has not been applied to this system. We report on the feasibility and biologic outcomes of a pilot study using alveolar and embryonal rhabdomyosarcoma xenograft lines..

Authors: Pediatr Blood Cancer. 2013 Mar;60(3):377-82. doi: 10.1002/pbc.24210. Epub 2012 Jun 12.
The application of radiation therapy to the Pediatric Preclinical Testing Program (PPTP): results of a pilot study in rhabdomyosarcoma.
Kaplon R, Hadziahmetovic M, Sommerfeld J, Bondra K, Lu L, Leasure J, Nguyen P, McHugh K, Li N, Chronowski C, Sebastian N, Singh M, Kurmasheva R, Houghton P, Pelloski CE.
Source:Wexner Medical Center at The Ohio State University, Arthur G. James Comprehensive Cancer Center and Richard L. Solove Research Institute, Columbus, Ohio 43210-1280, USA. 

USING NANODOT DOSIMETRY TO STUDY THE RS 2000 X-RAY BIOLOGICAL IRRADIATOR

Purpose: To use NanoDot dosimeters to study the RS 2000 X-ray Biological Irradiator dosimetry characteristics and perform in vivo dosimetry for cell or small animal experiments. .

Authors: 

Int J Radiat Biol. 2013 Jul 29. [Epub ahead of print]
Using NanoDot dosimetry to study the RS 2000 X-ray Biological Irradiator. Lu L, Bondra K, Gupta N, Sommerfeld J, Chronowski C, Leasure J, Singh M, Pelloski CE.

Source
Wexner Medical Center, The Arthur G. James Comprehensive Cancer Center and Richard J. Solove Research Institute, Department of Radiation Oncology, The Ohio State University , Columbus, Ohio , USA.

Website
https://www.ncbi.nlm.nih.gov/pubmed/23786571